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KMID : 0624620100430030164
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BMB Reports
2010 Volume.43 No. 3 p.164 ~ p.169
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The production and immunostimulatory activity of double-stranded CpG-DNA
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Park Byoung-Kwon
Kim Dong-Bum
Rhee Jae-Won
Kim Min-Soo
Seok Hyun-Jeong
Choi Soo-Young
Park Jin-Sook
Lee Young-Hee
Kwon Hyun-Joo
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Abstract
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CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence- independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.
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KEYWORD
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dsCpG-DNA, HLA-DR, IL-8, Innate immunity, Lipofectin
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